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聚合酶鏈反應(PCR)及反轉錄聚合酶鏈反應(RT-PCR)為分子生物學不可或缺的工具,可應用基因克隆、基因表達分析、診斷學等領域。PCR使用DNA引子,耐熱DNA聚合酶,藉由反覆的複製週期來生成大量目標DNA;而RT-PCR涉及使用反轉錄酵素將RNA反轉錄成互補DNA(cDNA),並使用PCR放大cDNA

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LEADSPHERE® One-Step RT-PCR Master Mix

Catalog Number:

LDG0027RF

Package:

Package:bulk

Description:

Description:Essential components for reliable RT-PCR are included in each single LEADSPHERE® One-Step RT-PCR Master Mix, which saves time for reaction set-up and reduces the risk of contamination and pipetting errors. In addition, the stabilized format facilitates shipment and storage without cooling. LEADSPHERE™ One-Step RT-PCR Master Mix includes reverse transcriptase, DNA polymerase, high-quality dNTPs, and optimized PCR buffer. This product is suitable for probe-based detection and endpoint DNA amplification. The reaction can be set up by reconstituting the LEADSPHERE™ One-Step RT-PCR Master Mix by adding PCR-grade water along with the RNA template, primers, and probe to a total volume of 20 μL.

LEADSPHERE® One-Step RT-PCR Master Mix

EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free)

Catalog Number:

LDG0032RF

Package:

Package:1 mL / Customized package

Description:

Description:EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free) is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.

  • Glycerol-free
EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free)

EffiStart™ 2X One-Step Probe RT-qPCR Master Mix (Glycerol-Free)

Catalog Number:

LDG0035RF

Package:

Package:1 mL / Customized package

Description:

Description:EffiStart™ 2X One-Step Probe RT-qPCR Master Mix (Glycerol-Free) is a one-step real-time reverse transcription-polymerase chain reaction (RT-qPCR) kit developed for cDNA synthesis and real-time PCR in the same tube. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the RNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.

  • Glycerol-free
EffiStart™ 2X One-Step Probe RT-qPCR Master Mix (Glycerol-Free)

EffiStart™ 5X One-Step Probe RT-qPCR Master Mix (Glycerol-Free)

Catalog Number:

LDG0036RF

Package:

Package:1 mL / Customized package

Description:

Description:EffiStart™ 5X One-Step Probe RT-qPCR Master Mix (Glycerol-Free) is a one-step real-time reverse transcription-polymerase chain reaction (RT-qPCR) kit developed for cDNA synthesis and real-time PCR in the same tube. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 5-fold premix. Reaction can be simply set up by adding the RNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.

  • Glycerol-free
EffiStart™ 5X One-Step Probe RT-qPCR Master Mix (Glycerol-Free)

EffiStart™ 2X Probe qPCR Master Mix (Glycerol-Free)

Catalog Number:

LDG0031RF

Package:

Package:1 mL / Customized package

Description:

Description:EffiStart™ 2X Probe qPCR Master Mix (Glycerol-Free) is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.

  • Glycerol-free
EffiStart™ 2X Probe qPCR Master Mix (Glycerol-Free)

M-MLV Reverse Transcriptase (Glycerol-Free)

Catalog Number:

LDG0011RF

Package:

Package:20,000 U / 50,000 U / Customized package

Description:

Description:Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase that synthesizes the first strand of complementary cDNA from a single-stranded RNA template with hybridized primer. This kit features high activity formulation of M-MLV RT and 5X reverse transcription buffer which are capable of full-length cDNA synthesis and high cDNA yields.

Expression system:

Expression system:Escherichia coli

  • Glycerol-free
M-MLV Reverse Transcriptase (Glycerol-Free)

dCas9-VP64

Catalog Number:

LDG003POE

Package:

Package:20 μg / 100 μg / Customized package

Species of Origin:

Species of Origin:Streptococcus pyogenes

Expression system:

Expression system:Escherichia coli

Affinity Tag:

Affinity Tag:His Tag (C-term)

  • Animal-free
  • Carrier-free
dCas9-VP64

eSpCas9 (1.1)

Catalog Number:

LDG002POE

Package:

Package:20 μg / 100 μg / Customized package

Species of Origin:

Species of Origin:Streptococcus pyogenes

Expression system:

Expression system:Escherichia coli

Affinity Tag:

Affinity Tag:His-MBP Tag (N-term)

  • Animal-free
  • Carrier-free
eSpCas9 (1.1)

wtCas9

Catalog Number:

LDG001POE

Package:

Package:5 μg / 20 μg / Customized package

Species of Origin:

Species of Origin:Streptococcus pyogenes

Expression system:

Expression system:Escherichia coli

Affinity Tag:

Affinity Tag:His Tag (C-term)

  • Animal-free
  • Carrier-free
wtCas9

Inorganic Pyrophosphatase (Yeast)

Catalog Number:

LDG0007RI

Package:

Package:10 U / Customized package

Description:

Description:Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P₂O₇⁻⁴ + H₂O + PPase → 2HPO₄⁻²). PPase requires divalent metal cation (Mg²⁺) for its enzymatic activity.

Expression system:

Expression system:Escherichia coli

  • Carrier-free
Inorganic Pyrophosphatase (Yeast)
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