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Leadgene Leadgene
Hot Start Taq DNA Polymerase (Glycerol-free)
Catalog Number

LDG0010RF

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  • Overview

    Description

    Leadgene® Hot Start Taq DNA Polymerase contains Taq DNA Polymerase and an anti-taq monoclonal antibody which blocks polymerase activity. Enzyme activity is recovered during the initial incubation step while the taq antibody is denatured and dissociates from the DNA polymerase. Hot Start Taq DNA Polymerase exhibits higher specificity, sensitivity, and yield by reducing non-specific amplification and primer-dimers. This enzyme possesses 5’→3’ polymerase activity and 5’ → 3’ exonuclease replacement activity, but lacks a 3’ → 5’ exonuclease (proof-reading) activity. Hot Start Taq DNA Polymerase is suitable for most PCR applications. The enzyme formulation does not contain glycerol and is compatible for further lyophilization process.

    Components

    Package

    Items

    Quantity

    500 U

    Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)

    1 vial (500 U)

    10× Hot Start Taq Buffer (Mg²⁺ free)

    1 vial (1 mL)

    25 mM MgCl₂

    1 vial (1 mL)

    1,000 U

    Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)

    1 vial (1,000 U)

    10× Hot Start Taq Buffer (Mg²⁺ free)

    2 vials (1 mL)

    25 mM MgCl₂

    2 vials (1 mL)

    • 500 U

      Items

      Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)

      Quantity

      1 vial (500 U)

      Items

      10× Hot Start Taq Buffer (Mg²⁺ free)

      Quantity

      1 vial (1 mL)

      Items

      25 mM MgCl₂

      Quantity

      1 vial (1 mL)

    • 1,000 U

      Items

      Hot Start Taq DNA Polymerase (Glycerol-Free) (5 U/μL)

      Quantity

      1 vial (1,000 U)

      Items

      10× Hot Start Taq Buffer (Mg²⁺ free)

      Quantity

      2 vials (1 mL)

      Items

      25 mM MgCl₂

      Quantity

      2 vials (1 mL)

  • Specifications
    • Application

      RT-PCR, qPCR

      Concentration

      5 U/µL

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Unit Definition

      One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.

    • Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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