聚合酶鏈反應(PCR)及反轉錄聚合酶鏈反應(RT-PCR)為分子生物學不可或缺的工具，可應用基因克隆、基因表達分析、診斷學等領域。PCR使用DNA引子，耐熱DNA聚合酶，藉由反覆的複製週期來生成大量目標DNA；而RT-PCR涉及使用反轉錄酵素將RNA反轉錄成互補DNA(cDNA)，並使用PCR放大cDNA。
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11 results
Inorganic Pyrophosphatase (Yeast)
Catalog Number：
Package：10 U / Customized package
Description：Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P₂O₇⁻⁴ + H₂O + PPase → 2HPO₄⁻²). PPase requires divalent metal cation (Mg²⁺) for its enzymatic activity.
Expression system：Escherichia coli
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Carrier-free
N1-Me-pseudo UTP Sodium Solution (100 mM)
Catalog Number：
Package：100 μL / Customized package
Description：N1-Me-Pseudo-UTP is a modified nucleotide that offers unique capabilities in studying RNA biology. By incorporating N1-Me-Pseudo-UTP into RNA molecules, researchers can introduce specific modifications and investigate their impact on RNA structure, function, and interactions. This powerful compound enables precise control over RNA modifications, allowing for detailed exploration of RNA processing, splicing, and translation. N1-Me-Pseudo-UTP is driving advancements in understanding RNA-related diseases and paving the way for targeted interventions and novel treatment strategies.
Pseudo UTP Sodium Solution (100 mM)
Catalog Number：
Package：100 μL / Customized package
Description：Pseudo UTP is a modified nucleotide that enables precise RNA modifications and analysis. With its unique structure, pseudo UTP allows for the incorporation of pseudouridine into RNA molecules, mimicking natural post-transcriptional modifications. This versatile compound has extensive applications in studying RNA structure, function, and interactions. Researchers can use pseudo UTP to explore the impact of pseudouridine on RNA stability, translation, and gene expression. By harnessing the potential of pseudo UTP, scientists are uncovering new insights into RNA biology, paving the way for advancements in therapeutics, diagnostics, and synthetic biology.
NTP Set, 100 mM Solutions
Catalog Number：
Package：1 mL*4 / Customized package
Description：The NTP Set consists of 4 separate 100 mM solutions of ATP, UTP, CTP, and GTP (pH 7.5) as sodium salts.
GTP Solution (100 mM)
Catalog Number：
Package：1 mL / Customized package
Description：Leadgene Guanosine-5'-triphosphate (GTP) is supplied as a 100 mM aqueous solution at pH 7.5.
CTP Solution (100 mM)
Catalog Number：
Package：1 mL / Customized package
Description：Leadgene Cytidine-5'-triphosphate (CTP) is supplied as a 100 mM aqueous solution at pH 7.5.
UTP Solution (100 mM)
Catalog Number：
Package：1 mL / Customized package
Description：Leadgene Uridine-5'-triphosphate (UTP) is supplied as a 100 mM aqueous solution at pH 7.5.
ATP Solution (100 mM)
Catalog Number：
Package：1 mL / Customized package
Description：Leadgene Adenosine 5'-triphosphate (ATP) is supplied as a 100 mM aqueous solution at pH 7.5.
T7 RNA Polymerase
Catalog Number：
Package：25,000 U / Customized package
Description：Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. This enzyme catalyzes the 5’→3’ synthesis of RNA from DNA downstream from its promoter.
Expression system：Escherichia coli
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Carrier-free
S-adenosylmethionine (SAM)
Catalog Number：
Package：50 μL * 10 vials (set) / bulk
Description：SAM (S-Adenosyl-L-methionine) is vital for cell function and survival, involved in metabolic pathways like transmethylation, transsulfuration, and polyamine synthesis. It also affects restriction enzyme cleavage. SAMe has been extensively studied for depression, osteoarthritis, and liver diseases, exploring its therapeutic benefits.
