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Leadgene Leadgene
wtCas9
Catalog Number

LDG001POE

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  • Specifications
    • Species of Origin

      Human

      Expression system

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 159.27 kDa.
      The protein migrates as 130-180 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      The wtCas9 is a recombinant Streptococcus pyogenes Cas9 protein (wild type form) purified from E. coli. Cas9 protein can form a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA). The RNP complex can recognizes the target site by matching gRNA with the genomic DNA sequence and make DNA breaks within 3 bases from the NGG PAM. With the CRISPR/Cas9 system, researchers can valid gene editing assay in cells or other kinds of animals.

      Synonyms

      CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9

    • Uniprot ID

      #Q99ZW2

      Sequence Note

      Met1-Asp1368

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant wtCas9.

    SDS-PAGE analysis of recombinant wtCas9.

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  • Datasheet & Documents
    MSDS
    Manual
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