Leadgene Leadgene
Inorganic Pyrophosphatase (Yeast)
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  • Overview


    Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P₂O₇⁻⁴ + H₂O + PPase → 2HPO₄⁻²). PPase requires divalent metal cation (Mg²⁺) for its enzymatic activity.

    Product Note

    Inorganic Pyrophosphatase (Yeast) requires divalent metal cation (Mg² ⁺) for its enzymatic activity. This enzyme is widely used in RNA IVT reaction.

  • Specifications
    • Expression system

      Escherichia coli


      In vitro transcription, RNA amplification, miRNA and siRNA synthesis

    • Concentration

      0.1 U/µL


      Inorganic pyrophosphatase is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and 50% glycerol.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Unit Definition

      One unit is defined as the amount of the enzyme hydrolysis 1 μmol of inorganic pyrophosphate in 1 minutes at 25°C.

    • Form


  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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