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Leadgene Leadgene
LEADSPHERE® One-Step RT-PCR Master Mix
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Catalog Number

LDG0027RF

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  • Overview

    Description

    Essential components for reliable RT-PCR are included in each single LEADSPHERE® One-Step RT-PCR Master Mix, which saves time for reaction set-up and reduces the risk of contamination and pipetting errors. In addition, the stabilized format facilitates shipment and storage without cooling. LEADSPHERE™ One-Step RT-PCR Master Mix includes reverse transcriptase, DNA polymerase, high-quality dNTPs, and optimized PCR buffer. This product is suitable for probe-based detection and endpoint DNA amplification. The reaction can be set up by reconstituting the LEADSPHERE™ One-Step RT-PCR Master Mix by adding PCR-grade water along with the RNA template, primers, and probe to a total volume of 20 μL.

    Product Note

    • Primer/Probe concentration

    Final concentrations of 400 nM (each primer) are suitable for most reactions. To obtain optimal condition, primer concentration can be titrated between 0.2-1 μM.
    A final concentration of 200 nM (probe) is suitable for most reactions. To obtain optimal condition, probe concentration can be titrated between 0.1-0.3 μM.

    • Annealing/Extension optimization

    To obtain optimal condition, annealing/extension temperature can be adjusted between 55°C-65°C, annealing/extension time can be extended up to 60 sec.

    • Target length

    Appropriate amplicon length should be arranged between 80-200 bp

  • Specifications
    • Application

      Reverse transcription, RT-PCR, qPCR

      Form

      Lyophilized

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 4-30°C for 12 months under sterile conditions from date of receipt.

      Keep dry.

  • Image
    Lyophilized LEADSPHERE® One-Step RT-PCR Master Mix

    Lyophilized LEADSPHERE® One-Step RT-PCR Master Mix

    Lyophilized LEADSPHERE® One-Step RT-PCR Master Mix

    Lyophilized LEADSPHERE® One-Step RT-PCR Master Mix

    Amplification of input RNA template in RT-qPCR assay. NTC: no template control

    Amplification of input RNA template in RT-qPCR assay.
    NTC: no template control

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  • Datasheet & Documents

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