Molecular Biology

Polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) are indispensable tools in molecular biology for amplifying specific DNA sequences. They are used in a variety of applications including gene cloning, gene expression analysis, and diagnostics. PCR utilizes short synthetic DNA primers, heat-stable DNA polymerase enzyme, and repeated cycles of replication to generate millions to billions of copies of the target DNA sequence. RT-PCR involves reverse transcription of RNA into complementary DNA (cDNA) using reverse transcriptase and amplification of the cDNA using PCR. Leadgene offers a comprehensive collection of high-quality PCR and RT-PCR reagents and master mix to ensure your results are accurate and sensitive.