Polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) are indispensable tools in molecular biology for amplifying specific DNA sequences. They are used in a variety of applications including gene cloning, gene expression analysis, and diagnostics. PCR utilizes short synthetic DNA primers, heat-stable DNA polymerase enzyme, and repeated cycles of replication to generate millions to billions of copies of the target DNA sequence. RT-PCR involves reverse transcription of RNA into complementary DNA (cDNA) using reverse transcriptase and amplification of the cDNA using PCR. Leadgene offers a comprehensive collection of high-quality PCR and RT-PCR reagents and master mix to ensure your results are accurate and sensitive.
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42 results
LEADSPHERE® Proteinase K
Catalog Number：
Package：50 tablets / Customized package
Description：Proteinase K is a non-specific serine protease that belongs to the subtilisin family with an active site catalytic triad Asp39-His69-Ser224. It is useful for the general digestion of peptide bonds, consequently used in broad applications in the biology experiments such as isolation of genomic DNA and plasmid, isolation of RNA, inactivation of RNases, DNases, and enzymes in reactions. LEADSPHERE® Proteinase K sphere is competent in the digestion of samples in 300-400 μL, such as saliva. In addition, LEADSPHERE® Proteinase K is generated with an ISO13485 quality management system specifically for medical devices.
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Glycerol-free
LEADSPHERE® One-Step RT-PCR Master Mix
Catalog Number：
Package：bulk
Description：Essential components for reliable RT-PCR are included in each single LEADSPHERE® One-Step RT-PCR Master Mix, which saves time for reaction set-up and reduces the risk of contamination and pipetting errors. In addition, the stabilized format facilitates shipment and storage without cooling. LEADSPHERE™ One-Step RT-PCR Master Mix includes reverse transcriptase, DNA polymerase, high-quality dNTPs, and optimized PCR buffer. This product is suitable for probe-based detection and endpoint DNA amplification. The reaction can be set up by reconstituting the LEADSPHERE™ One-Step RT-PCR Master Mix by adding PCR-grade water along with the RNA template, primers, and probe to a total volume of 20 μL.
EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free)
Catalog Number：
Package：1 mL / Customized package
Description：EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free) is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.
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Glycerol-free
EffiStart™ 2X One-Step Probe RT-qPCR Master Mix (Glycerol-Free)
Catalog Number：
Package：1 mL / Customized package
Description：EffiStart™ 2X One-Step Probe RT-qPCR Master Mix (Glycerol-Free) is a one-step real-time reverse transcription-polymerase chain reaction (RT-qPCR) kit developed for cDNA synthesis and real-time PCR in the same tube. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the RNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.
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Glycerol-free
EffiStart™ 5X One-Step Probe RT-qPCR Master Mix (Glycerol-Free)
Catalog Number：
Package：1 mL / Customized package
Description：EffiStart™ 5X One-Step Probe RT-qPCR Master Mix (Glycerol-Free) is a one-step real-time reverse transcription-polymerase chain reaction (RT-qPCR) kit developed for cDNA synthesis and real-time PCR in the same tube. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 5-fold premix. Reaction can be simply set up by adding the RNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.
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Glycerol-free
EffiStart™ 2X Probe qPCR Master Mix (Glycerol-Free)
Catalog Number：
Package：1 mL / Customized package
Description：EffiStart™ 2X Probe qPCR Master Mix (Glycerol-Free) is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.
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Glycerol-free
M-MLV Reverse Transcriptase (Glycerol-Free)
Catalog Number：
Package：20,000 U / 50,000 U / Customized package
Description：Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase that synthesizes the first strand of complementary cDNA from a single-stranded RNA template with hybridized primer. This kit features high activity formulation of M-MLV RT and 5X reverse transcription buffer which are capable of full-length cDNA synthesis and high cDNA yields.
Expression System：Escherichia coli
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Glycerol-free
dCas9-VP64
Catalog Number：
Package：20 μg / 100 μg / Customized package
Species of Origin：Streptococcus pyogenes
Expression System：Escherichia coli
Affinity Tag：His Tag (C-term)
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Animal-free
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Carrier-free
eSpCas9 (1.1)
Catalog Number：
Package：20 μg / 100 μg / Customized package
Species of Origin：Streptococcus pyogenes
Expression System：Escherichia coli
Affinity Tag：His-MBP Tag (N-term)
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Animal-free
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Carrier-free
