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Leadgene Leadgene
eSpCas9 (1.1)
Catalog Number

LDG002POE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His-MBP Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 201 kDa.
      The protein migrates about 180 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      The enhanced specificity Cas9 proteinfrom Streptococcus pyogenes (~160 KD), modified at sites K848A/K1003A/R1060A, exhibit substantially reduced off-target cleavage in cells. When combined with target RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and help the development of genetically-modified animals via one-cell embryo injection.

      Synonyms

      CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9

    • Uniprot ID

      #BDU59491.1

      Sequence Note

      Asp41-Asp1407

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant eSpCas9 (1.1).

    SDS-PAGE analysis of recombinant eSpCas9 (1.1).

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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