• Why should we add protective agent to the protein solution before lyophilization? Which kind of lyoprotectant do we usually add?
    Protective agents are used in freeze-drying and storage to stablize proteins. Commonly used lyoprotectant include trehalose and mannitol. Trehalose maintains protein secondary structure and prevents aggregation during lyophilization, while mannitol reduces the aggregation of lyophilized protein.