Leadgene Leadgene
EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free)
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  • Overview


    EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free) is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.

    Product Note

    • Primer/Probe concentration

    Final concentrations of 400 nM (each primer) are suitable for most reactions. To obtain optimal condition, primer concentration can be titrated between 0.2-1 μM.
    A final concentration of 200 nM (probe) is suitable for most reactions. To obtain optimal condition, probe concentration can be titrated between 0.1-0.3 μM.

    • Annealing/Extension optimization

    To obtain optimal condition, annealing/extension temperature can be adjusted between 55°C-65°C, annealing/extension time can be extended up to 60 sec.

    • Target length

    Appropriate amplicon length should be arranged between 80-200 bp.

  • Specifications
    • Application

      Reverse transcription, RT-PCR, qPCR



  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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