Language
TW
Leadgene Leadgene
Swine IL-15, His Tag, E. coli
Catalog Number

LDG015PSE

More Documents
Loading package & price...
  • Specifications
    • Species of Origin

      Swine

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 14.06 kDa.
      The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in TF-1 cells.
      The ED₅₀ for this effect is <5.5 ng/mL.

      Endotoxin Level

      <0.01 EU per 1 µg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 15 (IL-15) is a 12.65 kDa cytokine with 114 amino acid residues and is primarily produced from mononuclear phagocytes like dendritic cells, monocytes, and macrophages. IL-15 plays a critical role in the innate immune system when IL-15 binds to the IL-15 receptor. In addition, IL-15 mediates biological functions, such as the proliferation of natural killer cells and T cells, preventing the immune cells from apoptosis by activating STAT3 /JAK and PI3K/ALT signaling pathways.

      Synonyms

      Interleukin-15, IL-15

    • Uniprot ID

      #Q95253

      Sequence Note

      Thr49-Ser162

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant swine IL-15.

    SDS-PAGE analysis of recombinant swine IL-15.

    1
    /
    1
    Total   (1)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents
    MSDS
    Manual
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.