Protein 2D Electrophoresis

Two-dimensional gel electrophoresis (2D-gel) is a powerful technique that combine isoelectric focusing and SDS-PAGE to separate mixtures of proteins via their isoelectric point (pl) and molecular weight, respectively.

Leadgene offers a dedicated two-dimensional gel electrophoresis service to help you separate and analyze proteins from mixtures, which enables researchers to identify low-abundance proteins in samples. Besides, our capable project manager will support and discuss with you personally throughout the process.

• Key service features:
  • Compared to molecular weight-based protein separation method, 2D-gel technology has higher resolution in analyzing samples.
  • Leadgene offers the experienced scientist that help you successfully obtain the information of your needs.
  • We provide comprehensive data, which support you to analyze the mixtures of proteins.
• Service workflow:
  • Discuss the experimental details with client and understand the requirements.
  • Case confirmation and obtain the sample from client.
  • Protein quantitation
  • Proteins are first separated by their isoelectric point (pl) in isoelectric focusing.
  • Next, the proteins will be further resolved by SDS-PAGE that based on molecular weight.
  • Final report delivery
• Sample requirements:
  • Protein extraction
     Volume: ≤ 150 µL
     Concentration: ≥1 µg / µL (Silver stain), ≥2 µg / µL (Coomassie blue stain)
     To prevent the interference, salt and ionic detergent (eg. SDS) should not be in the presence of protein extraction buffer.
• ♦ Deliverables: Final report