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Leadgene Leadgene
Mouse G-CSF, His Tag, E. coli
Catalog Number

LDG004PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 19.76 kDa.
      The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in NFS-60 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant mouse G-CSF is > 2 x 10⁷ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Granulocyte Colony-Stimulating Factor (G-CSF) is a 18.67 kDa protein containing 207 amino acid which secreted by monocytes, macrophages, fibroblasts, and endothelial cells, regulates cell growth, maturation, and development of myeloid cells. G-CSF is a member of the CSF family of glycoproteins, and makes the bone marrow produce more white blood cells so it can reduce the risk of infection after some types of cancer treatment. G-CSF is also an established useful clinical agent for increasing neutrophilic granulocytes levels. Mouse G-CSF shares 73% sequence homology with human G-CSF.

      Synonyms

      Granulocyte colony-stimulating factor, G-CSF

    • Uniprot ID

      #P09920

      Sequence Note

      Glu55-Ala208

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse G-CSF.

    SDS-PAGE analysis of recombinant mouse G-CSF.

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  • Datasheet & Documents
    MSDS
    Manual
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