LEADSPHERE® Proteinase K

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Catalog Number

LDG0001RG

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• Overview

  Description

  Proteinase K is a non-specific serine protease that belongs to the subtilisin family with an active site catalytic triad Asp39-His69-Ser224. It is useful for the general digestion of peptide bonds, consequently used in broad applications in the biology experiments such as isolation of genomic DNA and plasmid, isolation of RNA, inactivation of RNases, DNases, and enzymes in reactions.
  LEADSPHERE® Proteinase K sphere is competent in the digestion of samples in 300-400 μL, such as saliva. In addition, LEADSPHERE® Proteinase K is generated with an ISO13485 quality management system specifically for medical devices.

  Product Note

  Please fine-tune the input sample volume to find the optimal condition for your assay.

  Components

  Package	Items	Quantity
  50 tablets	LEADSPHERE® Proteinase K	50 tablets
  	Proteinase K Diluent Buffer	1 vial (100 mL)

  • 50 tablets

    Items

    LEADSPHERE® Proteinase K

    Quantity

    50 tablets

    Items

    Proteinase K Diluent Buffer

    Quantity

    1 vial (100 mL)

• Specifications

  • Application

    Sample preparation

    Purity

    >95% as determined by SDS-PAGE analysis.

  • Reaction Condition

    One tablet for one reaction, and one reaction can digest 16 μg BSA incubated with
    Proteinase K for 1 hour at 37o C.

    Form

    Lyophilized

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 4-30°C for 12 months under sterile conditions from date of receipt.

    Keep dry.

• Image

  Lyophilized LEADSPHERE® Proteinase K

  Lyophilized LEADSPHERE® Proteinase K

  LEADSPHERE® Proteinase K pretreatment produces sensitive detection in RT-qPCR assay.

  LEADSPHERE™ Proteinase K completely digests bovine serum albumin (BSA).
  BSA was mixed with Proteinase K at 37 oC for an hour, and subsequently analyzed by SDS-PAGE. Lane 1. 2 μg of BSA Lane 2. 16 μg of BSA with 0.01 μg of Proteinase K Lane 3. 16 μg of BSA with 0.1 μg of Proteinase K Lane 4. 16 μg of BSA with 1 μg of Proteinase K

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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