Language
TW
Leadgene Leadgene
dCas9-VP64
Catalog Number

LDG003POE

More Documents
Loading package & price...
  • Specifications
    • Species of Origin

      Streptococcus pyogenes

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 169.29 kDa.
      The protein migrates as 130-180 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      dCas9 (deficient Cas9) protein is an endonuclease-inactive version of Cas9 (CRISPR associated protein 9). dCas9 lacks endonuclease activity so that it binds but does not cleave cognate DNA, which can be used to localize transcriptional activators or repressors to specific DNA sequences in order to control transcription.

      Synonyms

      CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9

    • Uniprot ID

      #PDB: 5Y36_A

      Sequence Note

      Met1-Asp1368

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant dCas9–VP64.

    SDS-PAGE analysis of recombinant dCas9–VP64.

    1
    /
    1
    Total   (1)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents
    MSDS
    Manual
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.