Animal-free reagent and laboratory
Manufactured and tested under GMP guideline
MSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN with polyhistidine tag at the C-terminus.
Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. Many different types of cells can produce IL-10, including immune cells and non-immune cells. IL-10 exerts inhibitory functions on DCs and macrophages, which is a potent inhibitor of antigen presentation and limit the production of the Th1-associated cytokines IL-2 and interferon-γ (IFN-γ). IL-10 is also a key immunoregulator during infection due to its inhibitory effect on inflammatory cytokine production. Consequently, the excessive Th1 and CD8+ T cell responses could be suppressed during infection.
Measure by its ability to induce MC/9‑2 cells proliferation.
The ED50 for this effect is <1 ng/mL.
The specific activity of recombinant human IL-10 is approximately >1 x106 IU/ mg.
Lyophilized protein should be stored at -20°C.
This product is stable for one year upon receipt, when handled and stored as instructed.
Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Avoid repeated freeze/thaw cycles.
1. David M. Mosser and Xia Zhang. Immunol Rev. (2008) 226: 205–218.
2. Ouyang W, O'Garra A. (2019) Immunity. 50,4: 871-891.
3. Gabryšová, L. et al. (2014) Curr Top Microbiol Immunol. 380: 157-90.
4. Kevin N. Couper et al. (2008) J Immunol.180 (9) 5771-5777.
*Carriers: 0.1% BSA, 5% HSA or 10% FBS.