Human TGF Beta 1, His Tag, E. coli

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Catalog Number

LDG051PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 13.7 kDa.
    The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to inhibit the IL-4 dependent proliferation in TF-1 cells. The ED₅₀ for this effect is < 0.1 ng/mL. The specific activity of recombinant human TGF beat 1 is approximately > 1 x 10⁷ IU/mg.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    The transforming Growth Factors beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions.

    Synonyms

    Transforming Growth Factors beta-1 proprotein

  • Uniprot ID

    #P01137

    Sequence Note

    Ala279-Ser390

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. In some experiments, it recommends to add 10 mM HCl when reconstitute lyophilized protein.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human TGF Beta 1, His Tag, E. coli (LDG051PHE) induced MCF-7 cell proliferation, with the ED50 at 3.13 ng/mL.

  SDS-PAGE analysis of recombinant human TGF beta 1.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.