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Leadgene Leadgene
Human IL-32 Alpha, His Tag, E. coli
Catalog Number

LDG036PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 15.72 kDa.
      The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce TNF alpha secretion in RAW264.7 cells. The ED₅₀ for this effect is <10 μg/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 32 alpha (IL-32α) is an 18 kDa cytokine with 131 amino acid residues and one of the IL-2 splice variants. IL-32α is primarily secreted from inflammatory cells, such as NK cells, T-cells, peripheral blood mononuclear cells (PBMCs), and monocytes. IL-32 alpha is a proinflammatory cytokine regulating IL-6 by interaction with protein kinase C(PKC). It also interacts with paxillin, integrin, and focal adhesion kinase 1 (FAK 1).

      Synonyms

      Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, Interleukin-31

    • Uniprot ID

      P24001-4

      Sequence Note

      Met1-Lys131

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human IL-32 Alpha.

    SDS-PAGE analysis of recombinant human IL-32 Alpha.

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  • Datasheet & Documents
    MSDS
    Manual
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