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Leadgene Leadgene
Human IL-12 (p40), His Tag, E. coli
Catalog Number

LDG015PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 35.64 kDa.
      The protein migrates as 45 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce cell proliferation in PHA-activated human peripheral blood lymphocytes (PBMC) using a concentration range of 5-50 ng/mL. Note: Results may vary from different PBMC donors.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 12 (IL-12) is a pro-inflammatory cytokine with a molecular weight of 70 kDa composed of two subunits, IL-12p35 (35 kDa) and IL-12p40 (40 kDa). IL-12p40 is induced in excess over the other subunits of IL-12 and IL-23 and can exist in a monomeric or homodimer form.

      Synonyms

      Interleukin-12 subunit beta, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, NKSF2

    • Uniprot ID

      #P29460

      Sequence Note

      Ile23-Ser328

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human IL-12 (p40).

    SDS-PAGE analysis of recombinant human IL-12 (p40).

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  • Datasheet & Documents
    MSDS
    Manual
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