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Species of Origin
Human
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 8.59 kDa.
The protein migrates as 8-10 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is 0.9-3.1 ng/mL. The specific activity of recombinant human IGF-I is approximately >1.2 x 10³ IU/mg.
Endotoxin Level
<0.01 EU per 1 µg of the protein by the LAL method.
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Form
Lyophilized
More Images
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Specifications
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Background
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Background
Insulin like Growth Factors 1 (IGF-I) is a 7.79 kDa member of the Insulin-like Growth Factors with 71 amino acid residues. IGF-I is mainly expressed from liver, adipose tissue, Endometrial stromal cells, Leydig cells, and can be isolated from plasma. IGF-I mediating the protein anabolic and promoting effect of pituitary growth hormone. IGF-I also affects metabolism of glycogen, DNA synthesis and glucose uptake via binding to IGF-I receptor.
Synonyms
Mechano Growth Factors, MGF, Somatomedin-C, Insulin-like Growth Factors
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Uniprot ID
P05019
Sequence Note
Gly49-Ala118
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![HPLC analysis of recombinant Human IGF-I, His Tag, E. coli. The purity of Human IGF-I Protein is greater than 95% as determined by RP-HPLC.]()
HPLC analysis of recombinant Human IGF-I, His Tag, E. coli.
The purity of Human IGF-I Protein is greater than 95% as determined by RP-HPLC.![Human IGF-I, His Tag, E. coli (LDG088PHE) induced MCF-7 cell proliferation, with the ED50 at 0.9084 ng/mL.]()
Human IGF-I, His Tag, E. coli (LDG088PHE) induced MCF-7 cell proliferation, with the ED50 at 0.9084 ng/mL.
![SDS-PAGE analysis of recombinant human IGF-I.]()
SDS-PAGE analysis of recombinant human IGF-I.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
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