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Leadgene Leadgene
Human IFN Omega, His Tag, E. coli
Catalog Number

LDG098PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 20.93 kDa.
      The protein migrates as 15-20 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce cytotoxicity in TF-1 cells. The ED₅₀ for this effect is <0.02 ng/mL. The specific activity of recombinant human IFN omega is approximately >5 x10⁷ IU/ mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interferon Omega (IFN-ω) is a 20.12 kDa member of type I IFN family with 173 amino acid residues. IL-28B is expressed by epithelial tissues. IFN-ω with antiviral, antitumor activity and regulating the innate immune response. Able to activate P13K/Akt signaling pathway via binding its receptor IFNAR in cells.

      Synonyms

      Interferon alpha-II-1, Interferon omega-1

    • Uniprot ID

      #P05000

      Sequence Note

      Cys24-Ser195

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human IFN omega.

    SDS-PAGE analysis of recombinant human IFN omega.

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  • Datasheet & Documents
    MSDS
    Manual
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