Human Galectin-10, His Tag, E. coli

Catalog Number

LDG147PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (N-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 16.31 kDa.
    The protein migrates as 16-19 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to chemoattract human THP1 cells using a concentration range of 0.2-25 µg/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Galectin-10 (Gal-10) is a lectin family member and is one of the prototype galectins. It contains one carbohydrate recognition domain (CRD), responsible for β-galactoside binding, and is biologically active as homodimers. It has been reported that galectin-10 is presented in eosinophils but also detected in basophils and macrophages. Accumulated evidence indicates that galectin-10 spontaneously forms Charcot-Leyden crystals (CLCs), participating in allergic responses and related inflammatory reactions. Several binding partners for galectin-10 have been identified, including eosinophil granule cationic ribonucleases, eosinophil-derived neurotoxin (EDN, RNS2), and eosinophil cationic protein (ECP, RNS3), demonstrating the interaction is essential for eosinophil differentiation and granulogenesis.

    Synonyms

    Gal-10, Charcot-Leyden crystal protein, CLC, Eosinophil lysophospholipase, Lysolecithin acylhydrolase

  • Uniprot ID

    #Q05315

    Sequence Note

    Ser2-Arg142

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human Galectin-10.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.