Leadgene Leadgene
Human FIXa, His Tag, CHO
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  • Specifications
    • Species of Origin


      Expression System


    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >95% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 49.54 kDa.
      The protein migrates as 50-60 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.



  • Background
    • Background

      FIXa (Factor IXa) protein is a serine protease involved in the coagulation cascade. Upon activation by Factor XIa or tissue factor-Factor VIIa complex, FIXa forms a complex with Factor VIIIa on phospholipid surfaces. This complex activates Factor X to Xa, ultimately leading to the formation of thrombin and fibrin clot formation. FIXa deficiency results in hemophilia B. Targeting FIXa activity is a therapeutic approach for controlling blood coagulation in hemophilia treatment. Understanding FIXa's role in hemostasis is crucial for developing hemophilia therapies and managing thrombotic disorders.


      Coagulation factor IX, Christmas factor, Plasma thromboplastin component (PTC)

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human FIXa.

    SDS-PAGE analysis of recombinant human FIXa.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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