Leadgene Leadgene
Human CNTF, His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 23.74 kDa.
      The protein migrates as 25 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in TF-1 cells. The ED₅₀ for this effect is <0.15 μg/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form


  • Background
    • Background

      Ciliary neurotrophic factor (CNTF) is the member of IL-6 cytokine family and expressed in the nervous system. CNTF is 22.9 kDa neurotrophic factor containing 200 residues, which shows multiple effects in vertebrate retinogenesis. Besides, CNTF acts as a promoter that not only accelerates adult neurogenesis but also increases the survival of neuron after injury. On the other hands, reducing number of hippocampal GABAergic interneurons, as well as 5-HT levels and 5-HT1A receptor expression has been found in CNTF knockout female mice, indicating that CNTF may influence affective behavior in females by regulation of neurotransmission.


      Ciliary neurotrophic factor, CNTF

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human CNTF.

    SDS-PAGE analysis of recombinant human CNTF.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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