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Human CD30L, His Tag, E. coli
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Catalog Number

LDG119PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 20.57 kDa.
      The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce IL-8 secretion in human PBMCs using a concentration range of 10 - 100 ng/mL.
      Note: Results may vary from different PBMC donors.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Human CD30L is also named for CD153 and TNFSF8 which is one member of TNF family. Human CD30L is expressed on T cells. It binds the CD30, which is on some lymphoma cell lines. Human CD30L is a 30 kDa cytokine with 171 amino acid residues which is a transmembrane glycoprotein. Human CD30L plays an important role in the regulation of cell proliferation, activation and apoptosis. Human CD30L is a good target of cell therapy in inflammatory diseases.

      Synonyms

      CD30 ligand , CD30L, Tumor necrosis factor ligand superfamily member 8

    • Uniprot ID

      P32971

      Sequence Note

      Gln63-Asp234

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Activity analysis of recombinant Human CD30L, His Tag, E. coli. Measure by its ability to induce IL-8 secretion in human PBMCs using a concentration range of 10 - 100 ng/mL. Note: Results may vary from different PBMC donors.

    Activity analysis of recombinant Human CD30L, His Tag, E. coli.
    Measure by its ability to induce IL-8 secretion in human PBMCs using a concentration range of 10 - 100 ng/mL.
    Note: Results may vary from different PBMC donors.

    SDS-PAGE analysis of recombinant human CD30L.

    SDS-PAGE analysis of recombinant human CD30L.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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