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Leadgene Leadgene
Human BMP-11, His Tag, E. coli
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Catalog Number

LDG109PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl and 50 mM L-arginine, pH 4.5.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 13.40 kDa.
      The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <11 ng/mL. Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is <4 ng/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Bone Morphogenetic Protein-11 (BMP-11), known as Growth differentiation factor 11 (GDF11), is an extracellular multifunctional signaling cytokine that is also a member of the TGFβ family. BMP-11 can bind with the TGFβ receptor and is involved in SMAD protein signal transduction. Moreover, it promotes the formation of blood vessels and nerves that can control anterior-posterior patterning.

      Synonyms

      Bone morphogenetic protein 11, Growth/differentiation factor 11

    • Uniprot ID

      #O95390

      Sequence Note

      Asn299-Ser407

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human BMP-11, His Tag, E. coli (LDG109PHE) induced alkaline phosphatase production by ATDC5 cells, with the ED50 at 10.93 ng/mL.

    Human BMP-11, His Tag, E. coli (LDG109PHE) induced alkaline phosphatase production by ATDC5 cells, with the ED50 at 10.93 ng/mL.

    SDS-PAGE analysis of recombinant human BMP-11.

    SDS-PAGE analysis of recombinant human BMP-11.

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  • Datasheet & Documents
    MSDS
    Manual
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