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Leadgene Leadgene
Anti-mCherry Antibody [Clone 45-5]
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Catalog Number

LDG0007YB

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  • Overview

    Description

    The mCherry protein is a basic red fluorescent protein derived from Discosoma sp. Leadgene mouse anti-mCherry mAb can recognize native and denature mCherry protein but not GFP.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IP: 1:500-2000
    IFA: 1:500-1000
    FACS: 1:500-1000

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2a

      Clone Name

      clone 45-5

    • Immunogen

      Recombinant mCherry protein

      Application

      ELISA, WB, IP, IFA, FACS

    • Conjugation

      Unconjugated

      Concentration

      1 mg/mL

    • Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

      Specificity

      Recognizes mCherry but not GFP.

    • Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of anti-mCherry mAb (1:500)

    Immunofluorescence analysis of anti-mCherry mAb (1:500)

    Flow cytometry analysis of anti-mCherry mAb (1:1000)

    Flow cytometry analysis of anti-mCherry mAb (1:1000)

    Western blotting analysis of anti-mCherry mAb (1:2000) Lane 1: 293T cell lysate Lane 2: 293T-Flag mCherry cell lysate Lane 3: GFP fusion protein Lysate/protein at 6 μg per lane

    Western blotting analysis of anti-mCherry mAb (1:2000)
    Lane 1: 293T cell lysate
    Lane 2: 293T-Flag mCherry cell lysate
    Lane 3: GFP fusion protein
    Lysate/protein at 6 μg per lane

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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