Anti-IL-1 Beta Antibody [Clone 4-1]

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Catalog Number

LDG0021YA

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• Overview

  Description

  Interleukin-1 is a group of proteins mainly produced by leukocytes, regulates inflammation via controlling a variety of innate immune processes. Interleukin-1 beta (IL-1 beta), a member of the IL-1 family, is a pro-inflammatory cytokine produced by macrophages. This cytokine plays a crucial role for host-response to infection and injury, and also is a important factor in cytokine storms.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:200-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2a

    Clone Name

    clone 4-1

  • Immunogen

    Interleukin 1 beta

    Reactivity

    Human

  • Application

    ELISA, WB, IFA, FACS

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Storage Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Specificity

    Interleukin 1 beta

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  ELISA titration of Anti-IL-1 Beta Antibody [Clone 4-1]
  Titration curve of anti-IL-1 Beta antibody in ELISA. Red: IL-1 Beta; Black: BSA (negative control).

  Immunofluorescence analysis of Anti-IL-1 beta Antibody [Clone 4-1]
  HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
  Triton X-100. Cells were stained with mouse anti-IL-1 beta monoclonal
  antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-
  iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342
  (Blue).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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