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Leadgene Leadgene
Anti-DENV pr Antibody [Clone 47-2]
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Catalog Number

LDG0013YA

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  • Overview

    Description

    Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. During dengue virus maturation, host protease furin cleaves prM to M and pr. The peripheral pr peptide is retained on the virus particle at acidic pH. This virus-pr complex does not bind target membranes and thus to inhibit virus-membrane interaction and blocks virus fusion.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:500-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG1

      Clone Name

      clone 47-2

    • Immunogen

      Dengue virus Pr

      Reactivity

      Dengue virus

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      pr

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Immunofluorescence analysis of Anti-DENV pr Antibody [Clone 47-2] Control BHK cells (uninfected) and Virus (DENV 2) infection BHK cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-DENV pr monoclonal antibody followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    Immunofluorescence analysis of Anti-DENV pr Antibody [Clone 47-2]
    Control BHK cells (uninfected) and Virus (DENV 2) infection BHK cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100.
    Cells were stained with mouse anti-DENV pr monoclonal antibody followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

    ELISA titration of Anti-DENV pr Antibody [Clone 47-2] Titration curve of anti-DENV pr antibody in ELISA. Red: pr; Black: BSA (negative control).

    ELISA titration of Anti-DENV pr Antibody [Clone 47-2]
    Titration curve of anti-DENV pr antibody in ELISA. Red: pr; Black: BSA (negative control).

    Western blotting analysis of Anti-DENV pr Antibody [Clone 47-2] Virus infection BHK cell lysates were stained with mouse anti-DENV pr monoclonal antibody at 1:1000 dilution. Lane 1: BHK cell lysate (10 μg), Lane 2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2 infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection (10 μg).

    Western blotting analysis of Anti-DENV pr Antibody [Clone 47-2]
    Virus infection BHK cell lysates were stained with mouse anti-DENV pr monoclonal antibody at 1:1000 dilution.
    Lane 1: BHK cell lysate (10 μg), Lane 2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2 infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection (10 μg).

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  • Datasheet & Documents
    MSDS
    Manual
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