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Catalog Number

LDG0041YA

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Overview

Description

The CXC chemokine is a group of secreted proteins, attracts neutrophils and lymphocytes. CXC chemokine ligand 12 (CXCL12 or Stromal cell-derived factor 1, SDF-1), a member of the CXC chemokines, exists in three alternative splicing variants (α, β and γ). This chemokine plays a crucial role in embryogenesis, hematopoiesis, angiogenesis and inflammation. CXCL12 is the only chemokine ligand for CXCR4, this ligand-receptor pair is essential during embryogenesis, hematopoiesis, and immune system organization.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-10000
IFA: 1:200-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG2a
  
  Clone Name
  
  clone 69-2
- Immunogen
  
  CXCL12
  
  Reactivity
  
  Human
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Storage Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  CXCL12
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-CXCL12 Antibody [Clone 69-2]
A549 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
Triton X-100. Cells were stained with mouse anti-CXCL12 monoclonal

antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-
iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342

(Blue).

FACS analysis of Anti-CXCL12 Antibody [Clone 69-2]
A549 cells were stained with mouse anti-CXCL12 monoclonal antibody at 2
μg/ml (red) and without antibody control (black).

ELISA titration of Anti-CXCL12 Antibody [Clone 69-2]
Titration curve of anti-CXCL12 antibody in ELISA. Red: CXCL12; Black: BSA
(negative control).

Western blotting analysis of Anti-CXCL12 Antibody [Clone 69-2]
HeLa cell lysates (30 μg) were stained with mouse anti-CXCL12 monoclonal
antibody at 1:5000 dilution.

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Datasheet & Documents

Datasheet

MSDS

Manual

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