Vaccinia Capping Enzyme

Catalog Number

LDG0013RI

Datasheet

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• Overview

  Description

  This capping enzyme contains three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase) to forming a Cap 0 structure (m7Gppp5'N). In the presence of the vaccine capping enzyme, GTP and SAM (methyl donor) the In Vitro Transcripts mRNA can be efficient add capped in less one hour.

  Product Note

  Reactionshould be performed under RNase free condition. Use nuclease-free tubes, reagents and water to avoid RNase contamination. Also, wear gloves when working with RNA.

  Components

  Package	Items	Quantity
  500 U	Vaccinia Capping Enzyme	1 vial (500 U)
  	10X Capping Buffer	1 vial (100 μL)

  • 500 U

    Items

    Vaccinia Capping Enzyme

    Quantity

    1 vial (500 U)

    Items

    10X Capping Buffer

    Quantity

    1 vial (100 μL)

• Specifications

  • Expression System

    Escherichia coli

    Application

    In vitro transcription, RNA amplification, miRNA and siRNA synthesis

  • Concentration

    10 U/µL

    Storage Buffer

    Vaccinia capping enzyme is supplied in 50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100 and 50% Glycerol.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Unit Definition

    One unit of Vaccinia Capping Enzyme is defined as the amount of enzyme required to incorporate 10 pmol of (α32P) GTP into an 80 nt transcript in 1 hour at 37°C

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.