Leadgene Leadgene
Swine IL-10, His Tag, E. coli
Catalog Number

LDG014PSE

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  • Specifications
    • Species of Origin

      Swine

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 19.05 kDa.
      The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce proliferation in MC/9‑2 cells. The ED₅₀ for this effect is <5 ng/mL.

      Endotoxin Level

      <0.01 EU per 1 µg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Interleukin 10 (IL-10) is a 17.54 kDa cytokine with 158 amino acid residues and is also known as human cytokine synthesis inhibitory factor (CSIF). IL-10 is an anti-inflammatory cytokine that is mainly secreted from monocytes, lymphocytes, and mast cells. IL-10 participates in the survival, proliferation, and antibody production of B cell via the JAK-STAT signaling pathway and suppress pro-inflammatory cytokine secretion by blocking the NF-κB signaling pathway.

      Synonyms

      Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF

    • Uniprot ID

      Q29055

      Sequence Note

      Ser19-Asn175

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant swine IL-10.

    SDS-PAGE analysis of recombinant swine IL-10.

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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