Species of Origin
His Tag (C-term)
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
>98% as determined by SDS-PAGE analysis.
The protein has a calculated MW of 18.51 kDa. The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis).
Measure by its ability to induce D10.G4.1 cells proliferation.
The ED₅₀ for this effect is <3 ng/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Interleukin-1 beta (IL-1β) is a major cytokine expressed in macrophage, NK cells, monocytes, and neutrophils. It also plays fundamental role in inflammatory response, including monocyte activation, which is essential for the host defense and pathogen resistance. Pro-IL-1β is cleaved by cytosolic caspase 1 to form mature IL-1β.
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
SDS-PAGE analysis of recombinant swine IL-1 beta.1/1
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Datasheet & Documents1/1
Disclaimer：For Research Use Only. Not for use in diagnostic or therapeutic procedures.