Affinity purified Streptavidin is conjugated to NHS-sepharose. It is an efficient technique for isolating biotinylated proteins. Streptavidin is a 52.8 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homotetramers have an extraordinarily high affinity for Biotin.
The Streptavidin sepharose can be used for affinity chromatography purifications assay development and immunoprecipitation. Streptavidin resins also can used in the physical separation of two DNA strands produced in a polymerase chain reaction by incorporating biotin in one of the amplification polymers.
The optimal protein concentration of the cell lysate should be determined by the end user.