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Leadgene Leadgene
Streptavidin Sepharose Purification Kit
Catalog Number

LDG0014RD

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  • Overview

    Description

    Affinity purified Streptavidin is conjugated to NHS-sepharose. It is an efficient technique for isolating biotinylated proteins. Streptavidin is a 52.8 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homotetramers have an extraordinarily high affinity for Biotin.
    The Streptavidin sepharose can be used for affinity chromatography purifications assay development and immunoprecipitation. Streptavidin resins also can used in the physical separation of two DNA strands produced in a polymerase chain reaction by incorporating biotin in one of the amplification polymers.

    Product Note

    The optimal protein concentration of the cell lysate should be determined by the end user.

  • Specifications
    • Application

      Immunoprecipitation, Protein purification

      Conjugation

      Sepharose

    • Specificity

      Streptavidin homotetramers have an extraordinarily high affinity for Biotin.

      Sample Type

      Cell lysate

    • Binding capacity

      The binding and elution capacity of 1 mL settled Streptavidin sepharose are commonly more than 5 mg of biotinylated protein.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product

      Please refer to product manual for storage constructions

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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