Leadgene® StablePlus™ 2X RT-LAMP Master Mix is an optimized master mix for reverse-transcription loop-mediated isothermal amplification (RT-LAMP) reactions. This product is a dual enzyme system, providing a rapid and sensitive detection in one pot. The amplified products can be detected by agarose gel electrophoresis.
The StablePlus™ version contains nucleic acid stabilizing agent to protect the amplified products.
- Primer concentration
Primer concentration can be titrated between 0.25X - 1X if undesired background signal appeared.
- Detection method
Both detecting the amplified products by agarose gel electrophoresis and turbidity changes due to magnesium pyrophosphate precipitation can be employed to analyze test results, but the latter is somehow less sensitive.