Leadgene Leadgene

ELISA Kit Development


Based out of Tainan City, Taiwan, Leadgene Biomedical specializes in generating and engineering antibodies and cytokines for IVD.

We produce high-quality antibody-related products in our ISO13485 certificated plant engaged in novel antibody discovery and manufacturing of patented antibody-related products, including raw materials, ELISA kits, and rapid tests. Along with our high-quality products, we provide excellent customized services, including pharmacokinetic measurement (Biacore T200), antibody development, and antibody engineering (hybridoma sequencing, antibody humanization, etc.)

Our team is energetic, diligent, trustworthy, and ambitious.

We will vigorously pursue innovation and continuously contribute to society.




The advantages of our outstanding ELISA kit development service:

  • Certification. We produce high-quality products in our ISO13485 certificated plant.
  • Customization. We offer a comprehensive analysis of antigen/antibody design.
  • High quality. We provide high specificity and high affinity antibodies.
  • Consultative service. A professional team discusses with you thoroughly to fulfill your need.
  • Profession. We provide excellent one-stop service to improve your performance.




The enzyme-linked immunosorbent assay (ELISA) is a commonly used method for antigen/antibody detection using a specific antibody (or antigen).

The quantitation is conducted by the signal of light absorption resulting from the enzymatic reaction.

There are several types of ELISA based on how the analytes and antibodies are bonded and used.


  • Direct ELISA. The enzyme-conjugated antibody directly binds to the target antigen (or antibody). The enzyme is triggered by the subtract and releases a specific wavelength of light.
  • Indirect ELISA. The targeting antibody is captured by a specific antigen immobilized on the microplate, and then an enzyme-conjugated secondary antibody is added to bind the target antibody for detection.
  • Sandwich ELISA. The targeting antigen is recognized by two specific antibodies. Because the detection needs two specific antibodies binding simultaneously, the sandwich ELISA shows high specificity.
  • Competitive ELISA. The targeting molecules compete with the standard molecules conjugated on the solid phase of the microplate for the binding of the specific antibodies. When the targeting antigen level in the sample is high, the level of antibody-bound standard antigen is low, and the color is lighter. The competitive ELISA is usually used to detect small molecules.


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