Reverse transcription (RT) and PCR can be performed as a one-step process by combining a reverse transcriptase and a DNA polymerase, allowing a specific and swift nucleic acid amplification using RNA as a template. This simple reaction requires a complex and optimized buffer system, and it can be accomplished with Leadgene’s innovative one-step RT-PCR master mix.
What are the benefits of Leadgene® One-Step RT-PCR Mix?
Trump the traditional two-step method, which requires converting RNA to cDNA before PCR amplification.
Compatible with LEADSPHERE® Lyophilization Technology for a lyophilized format (glycerol-free)
Optimized for quantitative PCR and isothermal PCR systems in various composition sets for different assay conditions: