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Leadgene Leadgene
SARS-CoV-2 Spike Protein (S1 Subunit), His Tag, E. coli
Catalog Number

LDG005PVE

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  • Specifications
    • Species of Origin

      SARS-CoV-2

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 74.34 kDa.
      The protein migrates as 63-75 kDa under reducing condition (SDS-PAGE analysis).

    • Form

      Lyophilized

  • Background
    • Background

      SARS‑CoV‑2 is a kind of coronavirus which full name is severe acute respiratory syndrome coronavirus 2. SARS‑CoV‑2 is contagious that causes the respiratory diseases and lung diseases which make difficulty breathing. SARS‑CoV‑2 do the spillover event in 2019 because it has genetic diversity. SARS-CoV-2 is composed by four subunits (spike, envelope, membrane and nucleocapsid proteins). Its RNA genome is encapsulated with nucleocapsid protein. The viral envelope is comprised of spike, envelope and membrane protein. SARS‑CoV‑2 has high affinity to ACE2, which is highly expression in intestines, kidney, and heart.

      Synonyms

      Spike glycoprotein, S glycoprotein, E2, Peplomer protein

    • Uniprot ID

      #P0DTC2

      Sequence Note

      Val16-Cys671

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant SARS-CoV-2 spike protein (S1 subunit).

    SDS-PAGE analysis of recombinant SARS-CoV-2 spike protein (S1 subunit).

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  • Publication
  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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