Leadgene Leadgene
SARS-CoV-2 Spike Protein (RBD), His Tag, E. coli
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  • Specifications
    • Species of Origin


      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 26.04 kDa.
      The protein migrates as 25-35 kDa under reducing condition (SDS-PAGE analysis).

    • Form


  • Background
    • Background

      Coronaviruses are enveloped positive-sense RNA viruses. Spike protein on the viral envelope and the cognate receptor on the surface of host cells that are essential for entry into host cells upon receptor binding and membrane fusion. Spike proteins are target for neutralization antibody, and mediate membrane fusion and virus entry. Receptor-binding domain (RBD), which is the S protein mediates viral entry into host cells by first binding to a host receptor.


      Spike glycoprotein, S glycoprotein, E2, Peplomer protein

    • Uniprot ID


      Sequence Note


  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.


      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant SARS-CoV-2 spike protein (RBD).

    SDS-PAGE analysis of recombinant SARS-CoV-2 spike protein (RBD).

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  • Publication
  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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