SARS-CoV-2 Nucleocapsid Protein, His-SUMO Tag, HEK293

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Catalog Number

LDG006PVM

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• Specifications

  • Species of Origin

    SARS-CoV-2

    Expression System

    HEK293 cell

  • Affinity Tag

    His-SUMO Tag (N-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 57.17 kDa.
    The protein migrates as 63-75 kDa under reducing condition (SDS-PAGE analysis).

  • Form

    Lyophilized

• Background

  • Background

    Coronaviruses (Delta B1.617.2 Variant) are enveloped positive-sense RNA viruses. Spike protein on the viral envelope and the cognate receptor on the surface of host cells that are essential for entry into host cells upon receptor binding and membrane fusion. Spike proteins are target for neutralization antibody, and mediate membrane fusion and virus entry.Trimeric spike protein is about 180 kDa, and contains two subunits, S1 and S2, mediating attachment and membrane fusion.

    Synonyms

    Nucleoprotein, N, Nucleocapsid protein, NC Protein N

  • Uniprot ID

    #P0DTC9

    Sequence Note

    Met1-Ala419

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Multi-cycle kinetic analysis of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG) to Human SARS-CoV-2 Nucleocapsid protein by using Biacore T200.

  ELISA titration of Human anti-SARS-CoV & CoV-2 NP Antibody (A. IgG; B. IgA; C. IgM antibody).

  WB analysis of recombinant Human SARS-CoV-2 nucleocapsid protein.
  Lane 1: Human anti-SARS-CoV & CoV-2 NP Antibody (IgG), 0.5 μg/mL (Leadgene cat. 17901)
  Lane 2: Human anti-SARS-CoV & CoV-2 NP Antibody (IgM), 0.5 μg/mL (Leadgene cat. 18301)

  SDS-PAGE analysis of recombinant Human SARS-CoV-2 nucleocapsid protein.

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• Review

  

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• Publication

  1. Chao CH et al. Frontiers in immunology. 2022; 13:1023943.
  2. Cheng YL et al. Frontiers in immunology. 2022; 13:941923.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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