Recombinant protein A were conjugated to NHS-sepharose. Protein A is a 42 kDa surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to human IgG, mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins. Therefore, the protein A sepharose is an efficient technique for IgG purification.
Recombinant protein A is immobilized at over 5 mg antibody per mL 50% slurry and this kit allows a rapid and efficient affinity purification of active IgG proteins. The affinity resin allows an efficient binding of IgG proteins without the need for preliminary steps and calibrations. The affinity bound IgG proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.
The optimal protein concentration of the cell lysate should be determined by the end user.