Leadgene Leadgene
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  • Overview


    Nuclease is a genetically engineered endonuclease from Serratia marcescens, it is produced and purified from E. coli. The enzyme is a non-specific nuclease, which effectively degrades single-stranded, double-stranded, linear, and circular DNA and RNA over a wide temperature and pH range.

  • Specifications
    • Expression System

      Escherichia coli


      250 U/μL

    • Storage Buffer

      Nuclease is supplied in 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 2 mM MgCl₂ and 50% glycerol


      >95% as determined by SDS-PAGE analysis.

    • Unit Definition

      One unit of Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA.



  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • -20°C for -80°Clong-term storage under sterile conditions.

      Avoid repeated free-thaw cycles.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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