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Leadgene Leadgene
Nuclease ELISA Kit
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Catalog Number

LDG00021E

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  • Overview

    Description

    Nuclease is a genetically engineered endonuclease from Serratia marcescens, it is produced and purified from E. coli. The enzyme is a non-specific nuclease, which effectively degrades single-stranded, double-stranded, linear, and circular DNA and RNA over a wide temperature and pH range.

    Components

    Nuclease ELISA plate

    96 wells (12 x 8-well strips)

    Standard

    1 vial (Lyophilized form)

    Standard reconstitution buffer

    1 vial (1.5 mL)

    HRP-antibody conjugate

    1 vial (70 μL)

    HRP-antibody conjugated diluent buffer

    1 vial (12 mL)

    20 X wash buffer

    1 vial (15 mL)

    TMB

    1 vial (12 mL)

    Stop solution

    1 vial (6 mL)

    Microplate sealing film

    1 sheet

    • Standard

      96 wells (12 x 8-well strips)

      1 vial (Lyophilized form)

    • Standard reconstitution buffer

      96 wells (12 x 8-well strips)

      1 vial (1.5 mL)

    • HRP-antibody conjugate

      96 wells (12 x 8-well strips)

      1 vial (70 μL)

    • HRP-antibody conjugated diluent buffer

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • 20 X wash buffer

      96 wells (12 x 8-well strips)

      1 vial (15 mL)

    • TMB

      96 wells (12 x 8-well strips)

      1 vial (12 mL)

    • Stop solution

      96 wells (12 x 8-well strips)

      1 vial (6 mL)

    • Microplate sealing film

      96 wells (12 x 8-well strips)

      1 sheet

  • Specifications
    • Reactivity

      Serratia marcescens

      Application

      Sandwich ELISA analysis

    • Sensitivity

      Limit of detection (LoD): 0.062 ng/mL.
      Limit of quantification (LoQ): 0.163 ng/mL.

      Specificity

      Nuclease

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only

    Typical data
    The following data are for demonstration only

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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