Description
Ni2+ were conjugated to NHS-sepharose beads.
Product Note
The optimal protein concentration of the cell lysate should be determined by the end user.
Description
Ni2+ were conjugated to NHS-sepharose beads.
Product Note
The optimal protein concentration of the cell lysate should be determined by the end user.
Application
Immunoprecipitation, Protein purification
Conjugation
Sepharose
Specificity
Recognizes recombinant proteins fused to the 6 x histidine (6 x His) tag expressed in bacteria, and insects.
Sample Type
Cell lysate
Form
Gel Immobilized 50% slurry in phosphate-buffered saline containing 0.05% sodium azide, pH 7.4.
Binding capacity
The binding and elution capacity of 1 mL sepharose are commonly more than 5 mg of recombinant 6X His fusion protein.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C.
Stability & Storage
This product is stable after storage at:
Do not freeze.
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Disclaimer:For Research Use or Further Manufacturing Only.