Leadgene Leadgene
Ni-NTA Sepharose
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  • Overview


    Ni2+ were conjugated to NHS-sepharose beads.

    Product Note

    The optimal protein concentration of the cell lysate should be determined by the end user.

  • Specifications
    • Application

      Immunoprecipitation, Protein purification



    • Specificity

      Recognizes recombinant proteins fused to the 6 x histidine (6 x His) tag expressed in bacteria, insects, and mammalian cells.

      Sample Type

      Cell lysate

    • Form

      Gel Immobilized 50% slurry in phosphate-buffered saline containing 0.05% sodium azide, pH 7.4.

      Binding capacity

      The binding and elution capacity of 1 mL sepharose are commonly more than 5 mg of recombinant 6X His fusion protein.

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for 12 months under sterile conditions from date of receipt.

      Do not freeze.

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  • Datasheet & Documents

Disclaimer:For Research Use or Further Manufacturing Only.

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