Description
Murine RNase Inhibitor effectively inhibits various RNases (RNase A, B, C). Its superior antioxidant activity, due to the absence of two oxidation-sensitive cysteine residues found in human inhibitors, makes it ideal for DTT-sensitive experiments like qPCR. Additionally, it is free from DNase, RNase, and Nickase contaminants, ensuring RNA sample integrity.
Expression System
Escherichia coli
Concentration
40 U/μL
Storage Buffer
20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% glycerol, pH 7.6
Purity
>95% as determined by SDS-PAGE analysis.
Unit Definition
One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5 ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
Form
Liquid
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
Stability & Storage
This product is stable after storage at:
Avoid repeated freeze/thaw cycles.
Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
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