mRNA Cap 2´-O-Methyltransferase

Catalog Number

LDG0014RI

Datasheet

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• Overview

  Description

  mRNA Cap 2´-O-Methyltransferase specifically requires a Cap 0 structure (m7Gppp5'N) as a substrate, which synthesis from in vitro transcripts mRNA and capping enzyme modify. This enzyme utilizes a methyl donor such as SAM to add a methyl group at the 2’-O position forming cap-1 structure.

  Product Note

  Reactionshould be performed under RNase free condition. Use nuclease-free tubes, reagents and water to avoid RNase contamination. Also, wear gloves when working with RNA.

  Components

  Package	Items	Quantity
  2 KU	mRNA Cap 2´-O-Methyltransferase	1 vial (2 KU)
  	10X Capping Buffer	1 vial (100 μL)

  • 2 KU

    Items

    mRNA Cap 2´-O-Methyltransferase

    Quantity

    1 vial (2 KU)

    Items

    10X Capping Buffer

    Quantity

    1 vial (100 μL)

• Specifications

  • Expression System

    Escherichia coli

    Application

    In vitro transcription, RNA amplification, miRNA and siRNA synthesis

  • Concentration

    50 U/µL

    Storage Buffer

    mRNA Cap 2;-O-Methyltransferase is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100 and 50% Glycerol.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Unit Definition

    One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.