Description
mRNA Cap 2´-O-Methyltransferase specifically requires a Cap 0 structure (m7Gppp5'N) as a substrate, which synthesis from in vitro transcripts mRNA and capping enzyme modify. This enzyme utilizes a methyl donor such as SAM to add a methyl group at the 2’-O position forming cap-1 structure.
Product Note
Reactionshould be performed under RNase free condition. Use nuclease-free tubes, reagents and water to avoid RNase contamination. Also, wear gloves when working with RNA.
Components
2 KU
Items
mRNA Cap 2´-O-Methyltransferase
Quantity
1 vial (2 KU)
Items
10X Capping Buffer
Quantity
1 vial (100 μL)
