Mouse Vimentin, His Tag, E. coli

Catalog Number

LDG094PME

Datasheet

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• Specifications

  • Species of Origin

    Mouse

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (N-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >95% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 54.5 kDa.
    The protein migrates as 50-55 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    Vimentin is an intermediate filament protein found in the cytoskeleton of eukaryotic cells, particularly abundant in mesenchymal cells. As a structural protein, vimentin provides mechanical support and stability to cells, contributing to their shape and motility. It plays a key role in various cellular processes, including cell adhesion, migration, and signaling. Additionally, vimentin is involved in regulating organelle positioning and maintaining cell integrity during stress and mechanical strain. Its expression is often associated with epithelial-to-mesenchymal transition (EMT) and is implicated in cancer metastasis and tissue remodeling. Understanding vimentin's functions and regulation is essential for elucidating its roles in both normal physiology and disease pathology.

    Synonyms

    Vim

  • Uniprot ID

    P20152 

    Sequence Note

    Met1-Glu466

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration of 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant mouse vimentin protein.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.