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Species of Origin
Mouse
Expression System
Escherichia coli
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Affinity Tag
His Tag (N-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 17.68 kDa.
The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce IL-2 secretion in mouse T cells in the presence of the anti-CD3 antibody. The ED₅₀ for this effect is <25 ng/mL.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
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Specifications
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Background
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Background
OX40L is a 16.86 kDa tumor necrosis factor family protein with 150 amino acid residues. OX40L is mainly expressed from lymphoid tissue, and promotes T-cell proliferation, survival, activation and cytokine production through receptor binding on the surface of T cells (OX40).
Synonyms
Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L
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Uniprot ID
#P43488
Sequence Note
Gln49-Leu198
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.