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Leadgene Leadgene
Mouse Midkine, His Tag, E. coli
Catalog Number

LDG088PME

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  • Specifications
    • Species of Origin

      Mouse

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 14.02 kDa.
      The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Form

      Lyophilized

  • Background
    • Background

      Midkine, also known as neurite growth-promoting factor 2 (NEGF2) is a member of a small family of secreted Growth Factorss, furthermore high expression in embryo, and limb E14.5.
      Mouse midkine shares 87% sequence identity with human midkine. Midkine is a 13.5 kDa protein containing 140 amino acids, which promotes angiogenesis, cell growth, migration, and gene expression of different cell types probably via a multiprotein receptor complex consisting of several molecules.

      Synonyms

      Midkine 1 Publication, MK, Retanoic acid-responsive protein, Retinoic acid-induced differentiation factor

    • Uniprot ID

      #P12025

      Sequence Note

      Gly31-Val119

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse Midkine.

    SDS-PAGE analysis of recombinant mouse Midkine.

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  • Datasheet & Documents
    MSDS
    Manual
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